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Transcriptome-wide interrogation of RNA secondary structure in living cells with icSHAPE
icSHAPE (in vivo click selective 2-hydroxyl acylation and profiling experiment) captures RNA secondary structure at a transcriptome-wide level by measuring nucleotide flexibility at base resolution. Living cells are treated with the icSHAPE chemical NAI-N(3) followed by selective chemical enrichment...
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| Udgivet i: | Nat Protoc |
|---|---|
| Main Authors: | , , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2016
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4896316/ https://ncbi.nlm.nih.gov/pubmed/26766114 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2016.011 |
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