Transcriptome-wide interrogation of RNA secondary structure in living cells with icSHAPE

icSHAPE (in vivo click selective 2-hydroxyl acylation and profiling experiment) captures RNA secondary structure at a transcriptome-wide level by measuring nucleotide flexibility at base resolution. Living cells are treated with the icSHAPE chemical NAI-N(3) followed by selective chemical enrichment...

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Gepubliceerd in:Nat Protoc
Hoofdauteurs: Flynn, Ryan A, Zhang, Qiangfeng Cliff, Spitale, Robert C, Lee, Byron, Mumbach, Maxwell R, Chang, Howard Y
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2016
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Article
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4896316/
https://ncbi.nlm.nih.gov/pubmed/26766114
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2016.011
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