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Creating new DNA binding specificities in the yeast transcriptional activator GCN4 by combining selected amino acid substitutions.

The specificity of the GCN4/DNA complex is mediated by a complicated network of interactions between the basic regions of both GCN4 monomers and their target halfsites. According to X-ray analyses (1, 2) one particular thymine of the target sequence is recognized by serine -11 and alanine -15 (we de...

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Dettagli Bibliografici
Autori principali: Suckow, M, Madan, A, Kisters-Woike, B, von Wilcken-Bergmann, B, Müller-Hill, B
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1994
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC523674/
https://ncbi.nlm.nih.gov/pubmed/8036145
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