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Protein Complex Affinity Capture from Cryomilled Mammalian Cells

Affinity capture is an effective technique for isolating endogenous protein complexes for further study. When used in conjunction with an antibody, this technique is also frequently referred to as immunoprecipitation. Affinity capture can be applied in a bench-scale and in a high-throughput context....

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:J Vis Exp
Hauptverfasser: LaCava, John, Jiang, Hua, Rout, Michael P.
Format: Artigo
Sprache:Inglês
Veröffentlicht: MyJove Corporation 2016
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5226390/
https://ncbi.nlm.nih.gov/pubmed/28060343
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54518
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