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Affinity proteomics to study endogenous protein complexes: Pointers, pitfalls, preferences and perspectives

Dissecting and studying cellular systems requires the ability to specifically isolate distinct proteins along with the co-assembled constituents of their associated complexes. Affinity capture techniques leverage high affinity, high specificity reagents to target and capture proteins of interest alo...

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Bibliografische gegevens
Gepubliceerd in:Biotechniques
Hoofdauteurs: LaCava, John, Molloy, Kelly R., Taylor, Martin S., Domanski, Michal, Chait, Brian T., Rout, Michael P.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2015
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4465938/
https://ncbi.nlm.nih.gov/pubmed/25757543
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000114262
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