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Affinity Purification of the RNA Degradation Complex, the Exosome, from HEK-293 Cells

The RNA exosome complex plays a central role in RNA processing and regulated turnover. Present both in cytoplasm and nucleus, the exosome functions through associations with ribonucleases and various adapter proteins (reviewed in [ Kilchert et al., 2016 ]). The following protocol describes an approa...

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Detalles Bibliográficos
Publicado en:Bio Protoc
Autores principales: Domanski, Michal, LaCava, John
Formato: Artigo
Lenguaje:Inglês
Publicado: Bio-Protocol 2017
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC5499702/
https://ncbi.nlm.nih.gov/pubmed/28691041
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2238
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