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Improved methodology for the affinity isolation of human protein complexes expressed at near endogenous levels

An efficient and reliable procedure for the capture of affinity-tagged proteins and associated complexes from human cell lines is reported. Through multiple optimizations, high yield and low background affinity-purifications are achieved from modest quantities of human cells expressing endogenous-le...

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Detalhes bibliográficos
Main Authors: Domanski, Michal, Molloy, Kelly, Jiang, Hua, Chait, Brian T., Rout, Michael P., Jensen, Torben Heick, LaCava, John
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3605737/
https://ncbi.nlm.nih.gov/pubmed/22668517
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000113864
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