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Improved methodology for the affinity isolation of human protein complexes expressed at near endogenous levels
An efficient and reliable procedure for the capture of affinity-tagged proteins and associated complexes from human cell lines is reported. Through multiple optimizations, high yield and low background affinity-purifications are achieved from modest quantities of human cells expressing endogenous-le...
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| Main Authors: | , , , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2012
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3605737/ https://ncbi.nlm.nih.gov/pubmed/22668517 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000113864 |
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