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Enzyme Kinetics for Complex System Enables Accurate Determination of Specificity Constants of Numerous Substrates in a Mixture by Proteomics Platform

Many important experiments in proteomics including protein digestion, enzyme substrate screening, enzymatic labeling, etc., involve the enzymatic reactions in a complex system where numerous substrates coexists with an enzyme. However, the enzyme kinetics in such a system remains unexplored and poor...

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Detalhes bibliográficos
Publicado no:Mol Cell Proteomics
Main Authors: Deng, Zhenzhen, Mao, Jiawei, Wang, Yan, Zou, Hanfa, Ye, Mingliang
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Biochemistry and Molecular Biology 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5217779/
https://ncbi.nlm.nih.gov/pubmed/27852747
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M116.062869
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