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Deep profiling of protease substrate specificity enabled by dual random and scanned human proteome substrate phage libraries

Proteolysis is a major posttranslational regulator of biology inside and outside of cells. Broad identification of optimal cleavage sites and natural substrates of proteases is critical for drug discovery and to understand protease biology. Here, we present a method that employs two genetically enco...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Zhou, Jie, Li, Shantao, Leung, Kevin K., O’Donovan, Brian, Zou, James Y., DeRisi, Joseph L., Wells, James A.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7568340/
https://ncbi.nlm.nih.gov/pubmed/32973096
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.2009279117
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