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Deep profiling of protease substrate specificity enabled by dual random and scanned human proteome substrate phage libraries
Proteolysis is a major posttranslational regulator of biology inside and outside of cells. Broad identification of optimal cleavage sites and natural substrates of proteases is critical for drug discovery and to understand protease biology. Here, we present a method that employs two genetically enco...
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| Udgivet i: | Proc Natl Acad Sci U S A |
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| Main Authors: | , , , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
2020
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7568340/ https://ncbi.nlm.nih.gov/pubmed/32973096 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.2009279117 |
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