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Deep profiling of protease substrate specificity enabled by dual random and scanned human proteome substrate phage libraries

Proteolysis is a major posttranslational regulator of biology inside and outside of cells. Broad identification of optimal cleavage sites and natural substrates of proteases is critical for drug discovery and to understand protease biology. Here, we present a method that employs two genetically enco...

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Publicat a:Proc Natl Acad Sci U S A
Autors principals: Zhou, Jie, Li, Shantao, Leung, Kevin K., O’Donovan, Brian, Zou, James Y., DeRisi, Joseph L., Wells, James A.
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2020
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7568340/
https://ncbi.nlm.nih.gov/pubmed/32973096
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.2009279117
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