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Deep profiling of protease substrate specificity enabled by dual random and scanned human proteome substrate phage libraries

Proteolysis is a major posttranslational regulator of biology inside and outside of cells. Broad identification of optimal cleavage sites and natural substrates of proteases is critical for drug discovery and to understand protease biology. Here, we present a method that employs two genetically enco...

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Pubblicato in:Proc Natl Acad Sci U S A
Autori principali: Zhou, Jie, Li, Shantao, Leung, Kevin K., O’Donovan, Brian, Zou, James Y., DeRisi, Joseph L., Wells, James A.
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 2020
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7568340/
https://ncbi.nlm.nih.gov/pubmed/32973096
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.2009279117
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