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Simultaneous Fluorescence In Situ Hybridization of mRNA and rRNA in Environmental Bacteria
We developed for Bacteria in environmental samples a sensitive and reliable mRNA fluorescence in situ hybridization (FISH) protocol that allows for simultaneous cell identification by rRNA FISH. Samples were carbethoxylated with diethylpyrocarbonate to inactivate intracellular RNases and pretreated...
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| Autores principales: | , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
American Society for Microbiology
2004
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC520857/ https://ncbi.nlm.nih.gov/pubmed/15345429 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.9.5426-5433.2004 |
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