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Simultaneous Fluorescence In Situ Hybridization of mRNA and rRNA in Environmental Bacteria

We developed for Bacteria in environmental samples a sensitive and reliable mRNA fluorescence in situ hybridization (FISH) protocol that allows for simultaneous cell identification by rRNA FISH. Samples were carbethoxylated with diethylpyrocarbonate to inactivate intracellular RNases and pretreated...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Pernthaler, Annelie, Amann, Rudolf
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2004
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC520857/
https://ncbi.nlm.nih.gov/pubmed/15345429
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.9.5426-5433.2004
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