Flow Sorting of Marine Bacterioplankton after Fluorescence In Situ Hybridization

We describe an approach to sort cells from coastal North Sea bacterioplankton by flow cytometry after in situ hybridization with rRNA-targeted horseradish peroxidase-labeled oligonucleotide probes and catalyzed fluorescent reporter deposition (CARD-FISH). In a sample from spring 2003 >90% of the...

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Detalhes bibliográficos
Main Authors: Sekar, Raju, Fuchs, Bernhard M., Amann, Rudolf, Pernthaler, Jakob
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
Assuntos:
Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC522093/
https://ncbi.nlm.nih.gov/pubmed/15466568
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.10.6210-6219.2004
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