Simultaneous Fluorescence In Situ Hybridization of mRNA and rRNA in Environmental Bacteria

We developed for Bacteria in environmental samples a sensitive and reliable mRNA fluorescence in situ hybridization (FISH) protocol that allows for simultaneous cell identification by rRNA FISH. Samples were carbethoxylated with diethylpyrocarbonate to inactivate intracellular RNases and pretreated...

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Pubblicato in:	Appl Environ Microbiol
Autori principali:	Pernthaler, Annelie, Amann, Rudolf
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 2004
Soggetti:	Methods
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC520857/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15345429/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.70.9.5426-5433.2004
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