Simultaneous Fluorescence In Situ Hybridization of mRNA and rRNA in Environmental Bacteria

We developed for Bacteria in environmental samples a sensitive and reliable mRNA fluorescence in situ hybridization (FISH) protocol that allows for simultaneous cell identification by rRNA FISH. Samples were carbethoxylated with diethylpyrocarbonate to inactivate intracellular RNases and pretreated...

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Dades bibliogràfiques

Publicat a:	Appl Environ Microbiol
Autors principals:	Pernthaler, Annelie, Amann, Rudolf
Format:	Artigo
Idioma:	Inglês
Publicat:	American Society for Microbiology (ASM) 2004
Matèries:	Methods
Accés en línia:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC520857/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15345429/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.70.9.5426-5433.2004
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