CRISPR/Cas9-mediated endogenous C-terminal Tagging of Trypanosoma cruzi Genes Reveals the Acidocalcisome Localization of the Inositol 1,4,5-Trisphosphate Receptor

Methods for genetic manipulation of Trypanosoma cruzi, the etiologic agent of Chagas disease, have been highly inefficient, and no endogenous tagging of genes has been reported to date. We report here the use of the CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR-asso...

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Vydáno v:J Biol Chem
Hlavní autoři: Lander, Noelia, Chiurillo, Miguel A., Storey, Melissa, Vercesi, Anibal E., Docampo, Roberto
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Biochemistry and Molecular Biology 2016
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5207250/
https://ncbi.nlm.nih.gov/pubmed/27793988
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M116.749655
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