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Probing conformational dynamics of an enzymatic active site by an in situ single fluorogenic probe under piconewton force manipulation

Unraveling the conformational details of an enzyme during the essential steps of a catalytic reaction (i.e., enzyme–substrate interaction, enzyme–substrate active complex formation, nascent product formation, and product release) is challenging due to the transient nature of intermediate conformatio...

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Bibliografski detalji
Izdano u:Proc Natl Acad Sci U S A
Glavni autori: Pal, Nibedita, Wu, Meiling, Lu, H. Peter
Format: Artigo
Jezik:Inglês
Izdano: National Academy of Sciences 2016
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5206548/
https://ncbi.nlm.nih.gov/pubmed/27940917
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1613404114
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