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Multiple- and single-molecule analysis of the actomyosin motor by nanometer-piconewton manipulation with a microneedle: unitary steps and forces.

We have developed a new technique for measurements of piconewton forces and nanometer displacements in the millisecond time range caused by actin-myosin interaction in vitro by manipulating single actin filaments with a glass microneedle. Here, we describe in full the details of this method. Using t...

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Autors principals: Ishijima, A, Kojima, H, Higuchi, H, Harada, Y, Funatsu, T, Yanagida, T
Format: Artigo
Idioma:Inglês
Publicat: 1996
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1224937/
https://ncbi.nlm.nih.gov/pubmed/8770215
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