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Two proofreading steps amplify the accuracy of genetic code translation

Aminoacyl-tRNAs (aa-tRNAs) are selected by the messenger RNA programmed ribosome in ternary complex with elongation factor Tu (EF-Tu) and GTP and then, again, in a proofreading step after GTP hydrolysis on EF-Tu. We use tRNA mutants with different affinities for EF-Tu to demonstrate that proofreadin...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Ieong, Ka-Weng, Uzun, Ülkü, Selmer, Maria, Ehrenberg, Måns
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5137768/
https://ncbi.nlm.nih.gov/pubmed/27837019
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1610917113
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