Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ

BACKGROUND: Although CRISPR/Cas enables one-step gene cassette knock-in, assembling targeting vectors containing long homology arms is a laborious process for high-throughput knock-in. We recently developed the CRISPR/Cas-based precise integration into the target chromosome (PITCh) system for a gene...

Descripció completa

Guardat en:
Dades bibliogràfiques
Publicat a:BMC Genomics
Autors principals: Aida, Tomomi, Nakade, Shota, Sakuma, Tetsushi, Izu, Yayoi, Oishi, Ayu, Mochida, Keiji, Ishikubo, Harumi, Usami, Takako, Aizawa, Hidenori, Yamamoto, Takashi, Tanaka, Kohichi
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2016
Matèries:
Methodology Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5126809/
https://ncbi.nlm.nih.gov/pubmed/27894274
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12864-016-3331-9
Etiquetes: Afegir etiqueta
Sense etiquetes, Sigues el primer a etiquetar aquest registre!

Ítems similars