Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice

Although the CRISPR/Cas system has enabled one-step generation of knockout mice, low success rates of cassette knock-in limit its application range. Here we show that cloning-free, direct nuclear delivery of Cas9 protein complex with chemically synthesized dual RNAs enables highly efficient target d...

詳細記述

保存先:
書誌詳細
出版年:Genome Biol
主要な著者: Aida, Tomomi, Chiyo, Keiho, Usami, Takako, Ishikubo, Harumi, Imahashi, Risa, Wada, Yusaku, Tanaka, Kenji F, Sakuma, Tetsushi, Yamamoto, Takashi, Tanaka, Kohichi
フォーマット: Artigo
言語:Inglês
出版事項: BioMed Central 2015
主題:
Method
オンライン･アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC4414275/
https://ncbi.nlm.nih.gov/pubmed/25924609
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-015-0653-x
タグ: タグ追加
タグなし, このレコードへの初めてのタグを付けませんか!

類似資料