Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice

Although the CRISPR/Cas system has enabled one-step generation of knockout mice, low success rates of cassette knock-in limit its application range. Here we show that cloning-free, direct nuclear delivery of Cas9 protein complex with chemically synthesized dual RNAs enables highly efficient target d...

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發表在:Genome Biol
Main Authors: Aida, Tomomi, Chiyo, Keiho, Usami, Takako, Ishikubo, Harumi, Imahashi, Risa, Wada, Yusaku, Tanaka, Kenji F, Sakuma, Tetsushi, Yamamoto, Takashi, Tanaka, Kohichi
格式: Artigo
語言:Inglês
出版: BioMed Central 2015
主題:
Method
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC4414275/
https://ncbi.nlm.nih.gov/pubmed/25924609
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-015-0653-x
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