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Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ
BACKGROUND: Although CRISPR/Cas enables one-step gene cassette knock-in, assembling targeting vectors containing long homology arms is a laborious process for high-throughput knock-in. We recently developed the CRISPR/Cas-based precise integration into the target chromosome (PITCh) system for a gene...
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| Pubblicato in: | BMC Genomics |
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| Autori principali: | , , , , , , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
BioMed Central
2016
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5126809/ https://ncbi.nlm.nih.gov/pubmed/27894274 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12864-016-3331-9 |
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