Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ

BACKGROUND: Although CRISPR/Cas enables one-step gene cassette knock-in, assembling targeting vectors containing long homology arms is a laborious process for high-throughput knock-in. We recently developed the CRISPR/Cas-based precise integration into the target chromosome (PITCh) system for a gene...

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Udgivet i:BMC Genomics
Main Authors: Aida, Tomomi, Nakade, Shota, Sakuma, Tetsushi, Izu, Yayoi, Oishi, Ayu, Mochida, Keiji, Ishikubo, Harumi, Usami, Takako, Aizawa, Hidenori, Yamamoto, Takashi, Tanaka, Kohichi
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2016
Fag:
Methodology Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5126809/
https://ncbi.nlm.nih.gov/pubmed/27894274
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12864-016-3331-9
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