Establishment of expanded and streamlined pipeline of PITCh knock-in – a web-based design tool for MMEJ-mediated gene knock-in, PITCh designer, and the variations of PITCh, PITCh-TG and PITCh-KIKO

The emerging genome editing technology has enabled the creation of gene knock-in cells easily, efficiently, and rapidly, which has dramatically accelerated research in the field of mammalian functional genomics, including in humans. We recently developed a microhomology-mediated end-joining-based ge...

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Bibliographic Details
Published in:Bioengineered
Main Authors: Nakamae, Kazuki, Nishimura, Yuki, Takenaga, Mitsumasa, Nakade, Shota, Sakamoto, Naoaki, Ide, Hiroshi, Sakuma, Tetsushi, Yamamoto, Takashi
Format: Artigo
Language:Inglês
Published: Taylor & Francis 2017
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Research Paper
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5470537/
https://ncbi.nlm.nih.gov/pubmed/28453368
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/21655979.2017.1313645
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