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G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay

Fӧrster resonance energy transfer (FRET)-based studies have become increasingly common in the investigation of GPCR signaling. Our research group developed an intra-molecular FRET sensor to detect the interaction between Gα subunits and GPCRs in live cells following agonist stimulation. Here, we det...

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Pubblicato in:J Vis Exp
Autori principali: Semack, Ansley, Malik, Rabia U., Sivaramakrishnan, Sivaraj
Natura: Artigo
Lingua:Inglês
Pubblicazione: MyJove Corporation 2016
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5092009/
https://ncbi.nlm.nih.gov/pubmed/27684955
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54696
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