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G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay
Fӧrster resonance energy transfer (FRET)-based studies have become increasingly common in the investigation of GPCR signaling. Our research group developed an intra-molecular FRET sensor to detect the interaction between Gα subunits and GPCRs in live cells following agonist stimulation. Here, we det...
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| Published in: | J Vis Exp |
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| Main Authors: | , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
MyJove Corporation
2016
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5092009/ https://ncbi.nlm.nih.gov/pubmed/27684955 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54696 |
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