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Protein–DNA footprinting by endcapped duplex oligodeoxyribonucleotides

Oligodeoxyribonucleotides (5′-phosphorylated) of varying lengths were capped using a polyamide linker to form thermodynamically stable, endcapped DNA duplexes containing 8–14 bp. We have employed these endcapped DNA duplexes as tools to determine the DNA footprint of T4 DNA ligase. By high-performan...

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Autors principals: Ng, Pei-Sze, Bergstrom, Donald E.
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2004
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC506826/
https://ncbi.nlm.nih.gov/pubmed/15263063
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh103
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