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Protein–DNA footprinting by endcapped duplex oligodeoxyribonucleotides

Oligodeoxyribonucleotides (5′-phosphorylated) of varying lengths were capped using a polyamide linker to form thermodynamically stable, endcapped DNA duplexes containing 8–14 bp. We have employed these endcapped DNA duplexes as tools to determine the DNA footprint of T4 DNA ligase. By high-performan...

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Detalhes bibliográficos
Main Authors: Ng, Pei-Sze, Bergstrom, Donald E.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC506826/
https://ncbi.nlm.nih.gov/pubmed/15263063
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh103
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