Simple cDNA normalization using kamchatka crab duplex-specific nuclease

We developed a novel simple cDNA normalization method [termed duplex-specific nuclease (DSN) normalization] that may be effectively used for samples enriched with full-length cDNA sequences. DSN normalization involves the denaturation–reassociation of cDNA, degradation of the double-stranded (ds) fr...

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Autors principals: Zhulidov, Pavel A., Bogdanova, Ekaterina A., Shcheglov, Alex S., Vagner, Laura L., Khaspekov, George L., Kozhemyako, Valery B., Matz, Mikhail V., Meleshkevitch, Ella, Moroz, Leonid L., Lukyanov, Sergey A., Shagin, Dmitry A.
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2004
Matèries:
NAR Methods Online
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC373426/
https://ncbi.nlm.nih.gov/pubmed/14973331
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh031
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