Simple cDNA normalization using kamchatka crab duplex-specific nuclease

We developed a novel simple cDNA normalization method [termed duplex-specific nuclease (DSN) normalization] that may be effectively used for samples enriched with full-length cDNA sequences. DSN normalization involves the denaturation–reassociation of cDNA, degradation of the double-stranded (ds) fr...

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Detaylı Bibliyografya
Asıl Yazarlar: Zhulidov, Pavel A., Bogdanova, Ekaterina A., Shcheglov, Alex S., Vagner, Laura L., Khaspekov, George L., Kozhemyako, Valery B., Matz, Mikhail V., Meleshkevitch, Ella, Moroz, Leonid L., Lukyanov, Sergey A., Shagin, Dmitry A.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Oxford University Press 2004
Konular:
NAR Methods Online
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC373426/
https://ncbi.nlm.nih.gov/pubmed/14973331
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh031
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