Simple cDNA normalization using kamchatka crab duplex-specific nuclease

We developed a novel simple cDNA normalization method [termed duplex-specific nuclease (DSN) normalization] that may be effectively used for samples enriched with full-length cDNA sequences. DSN normalization involves the denaturation–reassociation of cDNA, degradation of the double-stranded (ds) fr...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Zhulidov, Pavel A., Bogdanova, Ekaterina A., Shcheglov, Alex S., Vagner, Laura L., Khaspekov, George L., Kozhemyako, Valery B., Matz, Mikhail V., Meleshkevitch, Ella, Moroz, Leonid L., Lukyanov, Sergey A., Shagin, Dmitry A.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Oxford University Press 2004
Pynciau:
NAR Methods Online
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC373426/
https://ncbi.nlm.nih.gov/pubmed/14973331
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh031
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