Simple cDNA normalization using kamchatka crab duplex-specific nuclease

We developed a novel simple cDNA normalization method [termed duplex-specific nuclease (DSN) normalization] that may be effectively used for samples enriched with full-length cDNA sequences. DSN normalization involves the denaturation–reassociation of cDNA, degradation of the double-stranded (ds) fr...

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Detalles Bibliográficos
Publicado en:Nucleic Acids Res
Autores principales: Zhulidov, Pavel A., Bogdanova, Ekaterina A., Shcheglov, Alex S., Vagner, Laura L., Khaspekov, George L., Kozhemyako, Valery B., Matz, Mikhail V., Meleshkevitch, Ella, Moroz, Leonid L., Lukyanov, Sergey A., Shagin, Dmitry A.
Formato: Artigo
Lenguaje:Inglês
Publicado: Oxford University Press 2004
Materias:
NAR Methods Online
Acceso en línea:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC373426/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14973331/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnh031
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