Simple cDNA normalization using kamchatka crab duplex-specific nuclease

We developed a novel simple cDNA normalization method [termed duplex-specific nuclease (DSN) normalization] that may be effectively used for samples enriched with full-length cDNA sequences. DSN normalization involves the denaturation–reassociation of cDNA, degradation of the double-stranded (ds) fr...

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Bibliografiska uppgifter
Huvudupphovsmän: Zhulidov, Pavel A., Bogdanova, Ekaterina A., Shcheglov, Alex S., Vagner, Laura L., Khaspekov, George L., Kozhemyako, Valery B., Matz, Mikhail V., Meleshkevitch, Ella, Moroz, Leonid L., Lukyanov, Sergey A., Shagin, Dmitry A.
Materialtyp: Artigo
Språk:Inglês
Publicerad: Oxford University Press 2004
Ämnen:
NAR Methods Online
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC373426/
https://ncbi.nlm.nih.gov/pubmed/14973331
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh031
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