Bacterial Expression, Purification and In Vitro Phosphorylation of Full-Length Ribosomal S6 Kinase 2 (RSK2)

Ribosomal S6 kinases (RSK) play important roles in cell signaling through the mitogen-activated protein kinase (MAPK) pathway. Each of the four RSK isoforms (RSK1-4) is a single polypeptide chain containing two kinase domains connected by a linker sequence with regulatory phosphorylation sites. Here...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Utepbergenov, Darkhan, Hennig, Paulina M., Derewenda, Urszula, Artamonov, Mykhaylo V., Somlyo, Avril V., Derewenda, Zygmunt S.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2016
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5061434/
https://ncbi.nlm.nih.gov/pubmed/27732676
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0164343
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