Determination of the orientation of a DNA binding motif in a protein-DNA complex by photocrosslinking.
We have developed a straightforward biochemical method to determine the orientation of the DNA binding motif of a sequence-specific DNA binding protein relative to the DNA site in the protein-DNA complex. The method involves incorporation of a photoactivatable crosslinking agent at a single site wit...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1992
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC50323/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1332042/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.21.10287 |
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