Determination of the orientation of a DNA binding motif in a protein-DNA complex by photocrosslinking.

We have developed a straightforward biochemical method to determine the orientation of the DNA binding motif of a sequence-specific DNA binding protein relative to the DNA site in the protein-DNA complex. The method involves incorporation of a photoactivatable crosslinking agent at a single site wit...

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Detalles Bibliográficos
Main Authors: Pendergrast, P S, Chen, Y, Ebright, Y W, Ebright, R H
Formato: Artigo
Idioma:Inglês
Publicado: 1992
Assuntos:
Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC50323/
https://ncbi.nlm.nih.gov/pubmed/1332042
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