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Determination of the orientation of a DNA binding motif in a protein-DNA complex by photocrosslinking.

We have developed a straightforward biochemical method to determine the orientation of the DNA binding motif of a sequence-specific DNA binding protein relative to the DNA site in the protein-DNA complex. The method involves incorporation of a photoactivatable crosslinking agent at a single site wit...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Pendergrast, P S, Chen, Y, Ebright, Y W, Ebright, R H
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 1992
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC50323/
https://ncbi.nlm.nih.gov/pubmed/1332042
Tagiau: Ychwanegu Tag
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