Genome-wide quantification of rare somatic mutations in normal human tissues using massively parallel sequencing

We present the bottleneck sequencing system (BotSeqS), a next-generation sequencing method that simultaneously quantifies rare somatic point mutations across the mitochondrial and nuclear genomes. BotSeqS combines molecular barcoding with a simple dilution step immediately before library amplificati...

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Bibliographic Details
Published in:Proc Natl Acad Sci U S A
Main Authors: Hoang, Margaret L., Kinde, Isaac, Tomasetti, Cristian, McMahon, K. Wyatt, Rosenquist, Thomas A., Grollman, Arthur P., Kinzler, Kenneth W., Vogelstein, Bert, Papadopoulos, Nickolas
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 2016
Subjects:
Biological Sciences
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5024639/
https://ncbi.nlm.nih.gov/pubmed/27528664
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1607794113
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