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Simple, High-Yield Syntheses of DNA Duplexes Containing Interstrand DNA-DNA Cross-links Between an N(4)-Aminocytidine Residue and an Abasic Site

The protocol describes the preparation and purification of interstrand DNA-DNA cross-links derived from the reaction of an N(4)-aminocytidine residue with an abasic site in duplex DNA. The procedures employ inexpensive, commercially-available chemicals and enzymes to carry out post-synthetic modific...

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Detalhes bibliográficos
Publicado no:Curr Protoc Nucleic Acid Chem
Main Authors: Gamboa Varela, Jacqueline, Gates, Kent S.
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5000854/
https://ncbi.nlm.nih.gov/pubmed/27248783
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpnc.3
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