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Cell Fractionation and Quantitative Analysis of HIV-1 Reverse Transcription in Target Cells
This is a protocol to detect HIV-1 reverse transcription products in cytoplasmic and nuclear fractions of cells infected with VSV-G-pseudotyped envelope-defective HIV-1. This protocol can also be extended to HIV-1 with regular envelope.
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| Published in: | Bio Protoc |
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| Main Authors: | , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
2013
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4990143/ https://ncbi.nlm.nih.gov/pubmed/27547785 |
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