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Cell Fractionation and Quantitative Analysis of HIV-1 Reverse Transcription in Target Cells

This is a protocol to detect HIV-1 reverse transcription products in cytoplasmic and nuclear fractions of cells infected with VSV-G-pseudotyped envelope-defective HIV-1. This protocol can also be extended to HIV-1 with regular envelope.

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Detalhes bibliográficos
Publicado no:Bio Protoc
Main Authors: Shah, Vaibhav B, Aiken, Christopher
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4990143/
https://ncbi.nlm.nih.gov/pubmed/27547785
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