Increasing the efficiency of CRISPR/Cas9-mediated precise genome editing in rats by inhibiting NHEJ and using Cas9 protein

Precise modifications such as site mutation, codon replacement, insertion or precise targeted deletion are needed for studies of accurate gene function. The CRISPR/Cas9 system has been proved as a powerful tool to generate gene knockout and knockin animals. But the homologous recombination (HR)-dire...

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Bibliographic Details
Published in:RNA Biol
Main Authors: Ma, Yuanwu, Chen, Wei, Zhang, Xu, Yu, Lei, Dong, Wei, Pan, Shuo, Gao, Shan, Huang, Xingxu, Zhang, Lianfeng
Format: Artigo
Language:Inglês
Published: Taylor & Francis 2016
Subjects:
Brief Communication
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4962809/
https://ncbi.nlm.nih.gov/pubmed/27163284
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/15476286.2016.1185591
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