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Increasing the efficiency of CRISPR/Cas9-mediated precise genome editing in rats by inhibiting NHEJ and using Cas9 protein

Precise modifications such as site mutation, codon replacement, insertion or precise targeted deletion are needed for studies of accurate gene function. The CRISPR/Cas9 system has been proved as a powerful tool to generate gene knockout and knockin animals. But the homologous recombination (HR)-dire...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:RNA Biol
Hauptverfasser: Ma, Yuanwu, Chen, Wei, Zhang, Xu, Yu, Lei, Dong, Wei, Pan, Shuo, Gao, Shan, Huang, Xingxu, Zhang, Lianfeng
Format: Artigo
Sprache:Inglês
Veröffentlicht: Taylor & Francis 2016
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4962809/
https://ncbi.nlm.nih.gov/pubmed/27163284
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/15476286.2016.1185591
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