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Beyond editing: repurposing CRISPR–Cas9 for precision genome regulation and interrogation

The bacterial CRISPR–Cas9 system has emerged as a multifunctional platform for sequence-specific regulation of gene expression. This Review describes the development of technologies based on nuclease-deactivated Cas9, termed dCas9, for RNA-guided genomic transcription regulation, both by repression...

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Detalhes bibliográficos
Publicado no:Nat Rev Mol Cell Biol
Main Authors: Dominguez, Antonia A., Lim, Wendell A., Qi, Lei S.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4922510/
https://ncbi.nlm.nih.gov/pubmed/26670017
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nrm.2015.2
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