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Directed evolution of RebH for catalyst-controlled halogenation of indole C–H bonds
RebH variants capable of chlorinating substituted indoles ortho-, meta-, and para- to the indole nitrogen were evolved by directly screening for altered selectivity on deuterium-substituted probe substrates using mass spectrometry. This systematic approach allowed for rapid accumulation of beneficia...
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| Publicado no: | Chem Sci |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Royal Society of Chemistry
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4917012/ https://ncbi.nlm.nih.gov/pubmed/27347367 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c5sc04680g |
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