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Improving the Stability and Catalyst Lifetime of the Halogenase RebH Using Directed Evolution
We previously reported that the halogenase RebH catalyzes selective halogenation of several heterocycles and carbocycles, but product yields were limited by enzyme instability. Here, we use directed evolution to engineer a RebH variant, 3-LR, with a T(opt) over 5 °C higher than that of wild type, an...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4124618/ https://ncbi.nlm.nih.gov/pubmed/24849696 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201300780 |
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