Selective 2′-hydroxyl acylation analyzed by primer extension and mutational profiling (SHAPE-MaP) for direct, versatile, and accurate RNA structure analysis

SHAPE chemistries exploit small electrophilic reagents that react with the 2′-hydroxyl group to interrogate RNA structure at single-nucleotide resolution. Mutational profiling (MaP) identifies modified residues based on the ability of reverse transcriptase to misread a SHAPE-modified nucleotide and...

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Publicado en:Nat Protoc
Autores principales: Smola, Matthew J., Rice, Greggory M., Busan, Steven, Siegfried, Nathan A., Weeks, Kevin M.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2015
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Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4900152/
https://ncbi.nlm.nih.gov/pubmed/26426499
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2015.103
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