Selective 2′-Hydroxyl Acylation analyzed by Protection from Exoribonuclease (RNase-detected SHAPE): direct analysis of covalent adducts and of nucleotide flexibility in RNA

RNA SHAPE chemistry yields quantitative, single nucleotide resolution structural information based on the reaction of the 2′-hydroxyl group of conformationally flexible nucleotides with electrophilic SHAPE reagents. However, SHAPE technology has been limited by the requirement that sites of RNA modi...

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Publicado en:Nat Protoc
Autores principales: Steen, Kady-Ann, Siegfried, Nathan A., Weeks, Kevin M.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2011
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Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4940041/
https://ncbi.nlm.nih.gov/pubmed/21979276
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2011.373
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