Site-specific protein labeling with PRIME and chelation-assisted Click chemistry

This protocol describes an efficient method to site-specifically label cell-surface or purified proteins with chemical probes in two steps: PRobe Incorporation Mediated by Enzymes (PRIME) followed by chelation-assisted copper-catalyzed azide-alkyne cycloaddition (CuAAC). In the PRIME step, Escherich...

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Veröffentlicht in:Nat Protoc
Hauptverfasser: Uttamapinant, Chayasith, Sanchez, Mateo I., Liu, Daniel S., Yao, Jennifer Z., White, Katharine A., Grecian, Scott, Clarke, Scott, Gee, Kyle R., Ting, Alice Y.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2013
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4892701/
https://ncbi.nlm.nih.gov/pubmed/23887180
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2013.096
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