Site-specific protein labeling with PRIME and chelation-assisted Click chemistry

This protocol describes an efficient method to site-specifically label cell-surface or purified proteins with chemical probes in two steps: PRobe Incorporation Mediated by Enzymes (PRIME) followed by chelation-assisted copper-catalyzed azide-alkyne cycloaddition (CuAAC). In the PRIME step, Escherich...

Descripción completa

Guardado en:
Detalles Bibliográficos
Publicado en:Nat Protoc
Autores principales: Uttamapinant, Chayasith, Sanchez, Mateo I., Liu, Daniel S., Yao, Jennifer Z., White, Katharine A., Grecian, Scott, Clarke, Scott, Gee, Kyle R., Ting, Alice Y.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2013
Materias:
Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4892701/
https://ncbi.nlm.nih.gov/pubmed/23887180
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2013.096
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!

Ejemplares similares