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A fluorophore ligase for site-specific protein labeling inside living cells

Biological microscopy would benefit from smaller alternatives to green fluorescent protein for imaging specific proteins in living cells. Here we introduce PRIME (PRobe Incorporation Mediated by Enzymes), a method for fluorescent labeling of peptide-fused recombinant proteins in living cells with hi...

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Autors principals: Uttamapinant, Chayasith, White, Katharine A., Baruah, Hemanta, Thompson, Samuel, Fernández-Suárez, Marta, Puthenveetil, Sujiet, Ting, Alice Y.
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2010
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2890758/
https://ncbi.nlm.nih.gov/pubmed/20534555
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0914067107
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