RAC-tagging: Recombineering And Cas9-assisted targeting for protein tagging and conditional analyses

A fluent method for gene targeting to establish protein tagged and ligand inducible conditional loss-of-function alleles is described. We couple new recombineering applications for one-step cloning of gRNA oligonucleotides and rapid generation of short-arm (~1 kb) targeting constructs with the power...

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Published in:Sci Rep
Main Authors: Baker, Oliver, Gupta, Ashish, Obst, Mandy, Zhang, Youming, Anastassiadis, Konstantinos, Fu, Jun, Stewart, A. Francis
Format: Artigo
Language:Inglês
Published: Nature Publishing Group 2016
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4877586/
https://ncbi.nlm.nih.gov/pubmed/27216209
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep25529
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